Safety Features. Name Rating Plate. Rotor Chamber. Refrigeration System. Controls and Indicators. Control Panel. Space and Location Requirements. Run Procedures. Preparation and Loading. Starting a Run. Stopping a Run. General Maintenance. Sterilization and Disinfection. Tube Breakage. Speed Calibration. Speed Verification. Speed Knob Adjustment. Temperature Knob Adjustment.
Storage and Transport. Returning a Centrifuge. Supply List. Replacement Parts. Allegra 6 Centrifuges. Interior View of the Centrifuge. The Control Panel Nonrefrigerated Model. The Control Panel Refrigerated Models. Centrifuge Dimensions. Emergency Access. They have been designed and tested to be compliant when used with Beckman Coulter rotors with the laboratory equipment requirements of applicable regulatory agencies.
Declarations of conformity and certificates of compliance are available at www. References in this manual to an Allegra 6R model also apply to the Spinchron R model. NOTE If the centrifuge is used in a manner other than specified in this manual, the safety and performance of this equipment could be impaired.
Further, the use of any equipment other than that recommended by Beckman Coulter has not been evaluated for safety. These international symbols may also be displayed on the centrifuge and are reproduced and described below and on the inside of the front cover.
NOTE Used to call attention to important information that should be followed during installation, use, or servicing of this equipment. It is also used to alert against unsafe practices. Mechanical damage may also result. Refer servicing of all areas displaying either symbol to service personnel. To ensure minimal environmental impact, no CFCs are used in the manufacture or operation of Allegra 6 series and Spinchron R centrifuges. These limits are designed to provide reasonable protection against harmful interference when the equipment is operated in a commercial environment.
This equipment generates, uses, and can radiate radio frequency energy and, if not installed and used in accordance with this instruction manual, may cause interference to radio communications. Operation of this equipment in a residential area is likely to cause interference, in which case the user will be required to correct the interference at his own expense. The presence of this marking on the product indicates. It is very important that customers understand and follow all laws regarding the proper decontamination and safe disposal of electrical equipment.
For Beckman Coulter products bearing this label please contact your dealer or local Beckman Coulter office for details on the take back program that will facilitate the proper collection, treatment, recovery, recycling and safe disposal of the device.
Values without tolerances are informative data, without guarantee. Allegra 6. Dimensions Width. Depth overall. Depth at base. Height, door closed. Height, door open. Electrical requirements. Class I. Class I Maximum heat dissipation into room under steady-state conditions.
II Pollution degree. Safety features include the following. When the door is closed it locks automatically. Two independent monitoring systems prevent the door from opening if the rotor is spinning.
At low speeds, an incorrectly loaded rotor can cause imbalance. Rotor instability can also occur if the centrifuge is moved while running, or if it is not resting on a level surface. The anchoring system bolts that anchor a benchtop centrifuge to the laboratory bench; a stationary tray to roll a kneewell centrifuge into for operation is designed to minimize the possibility of injury or damage that could result from centrifuge movement in the event of a major rotor mishap. Check that the line voltage agrees with the voltage listed on this name rating plate before connecting the centrifuge.
Always mention the serial number and the model number shown when corresponding with Beckman Coulter regarding your centrifuge the serial number is also on the inside of the door. The control panel overlay is made of coated polycarbonate. Polyvinyl chloride and polyester foam panels line the interior walls of the centrifuge housing for sound and temperature insulation.
DOOR The structural steel door has a strobe port for speed verification. In nonrefrigerated models, the door has two filtered air-intake ports for rotor cooling. An electromechanical door lock system, together with a manual lock, prevents run initiation unless the door is closed and latched. When a run is in progress, the door locks automatically and can not be opened unless the power is on, the rotor is virtually stopped spinning less than 40 rpm , and the manual lock is in the UNLOCK position.
The drive shaft and neoprene rubber boot surrounding the drive shaft are visible in the chamber bottom. A neoprene gasket around the chamber top assures sealing. Centrifuge gaskets have not been designed as bioseals for aerosol containment. The thermistor is also visible in refrigerated models. Nonrefrigerated models only—During centrifugation, the rotor movement draws air through the filtered air-intake ports in the door into the chamber bowl, where it circulates around the rotor.
Holes in the side of the bowl allow air to flow out to the exhaust ports beneath the centrifuge. This air flow provides cooling for the rotor.
A tie-down screw or rotor knob secures the rotor to the drive shaft. The resilient suspension ensures that loads will not be disturbed by vibration, and prevents damage to the drive shaft if an imbalance occurs during centrifugation.
NOTE To avoid chamber icing, refrigeration is off when the door is open. The centrifuge door must be closed and locked for the refrigeration system to begin operating. The switch lights up when the power is on. When pressed to OPEN, this toggle switch releases the latch mechanism and allows the door to be opened the rotor must be stopped, the power must be on, and the manual lock in UNLOCK position.
This switch is used to select the brake mode used during deceleration. Refer to the appropriate rotor manual for acceleration and deceleration times for available rotors under various conditions. When rotor speed reaches 50 rpm, all braking stops and the rotor coasts to a stop. This variable-setting knob may be set for timed runs or longerduration continuous runs.
Either type of run can be stopped at any time by switching the timer to OFF. The variable-setting knob is used to set the drive speed, in rpm increments, up to rpm. The centrifuge will accelerate to the set speed and maintain that speed for the duration of the run. Never bring any flammable substances within the cm 1-ft area surrounding the centrifuge. Maintain a clearance envelope of 7. No persons should be within this clearance envelope while the centrifuge is operating. Save the container and packing materials for possible future relocation or storage of the centrifuge.
Dimensions are shown in Figure The centrifuge must have adequate air ventilation to ensure compliance to local requirements for vapors produced during operation. Use the appropriate anti-rotation kit to secure the centrifuge to the working surface. The anti-rotation kit is designed to minimize the possibility of injury or damage that could result from centrifuge movement in the event of a rotor mishap.
Complete instructions SP-TB for installing the anti-rotation kit are included with the kit, which is shipped with the centrifuge. Complete instructions GS6-TB for installing the anti-rotation kit are included with the kit, which is shipped with the centrifuge. In regions where a different plug is required to meet local electrical and safety requirements, contact your local Beckman Coulter representative.
Make sure the voltage imprinted on the name rating plate affixed to the back of the centrifuge agrees with the line voltage of the outlet used. If there is any question about voltage, have a qualified service person measure it under load while the drive is operating. To reduce the risk of electrical shock, this equipment uses a three-wire electrical cord and plug to connect the centrifuge to earth-ground.
To ensure safety, the centrifuge should be wired to a remote emergency switch preferably outside the room where the centrifuge is housed, or adjacent to the exit from that room , in order to disconnect the centrifuge from the main power source in case of a malfunction. NOTE During transport between areas with varying temperatures, condensation may occur inside the centrifuge. Allow sufficient drying time before running the centrifuge. We recommend that you make a test run to ensure that the centrifuge is in proper operating condition after shipment.
See Section 3 for instructions on operating the centrifuge. After completing the test run, return the preaddressed warranty card included with this literature. WARNING The centrifuge must not be used in the vicinity of flammable liquids or vapors, and such materials should not be run in the centrifuge.
During operation you should not come within the 7. Do not lean on the centrifuge or place items on the centrifuge while it is operating. Refer to the appropriate rotor manual for instructions on preparing the rotor for centrifugation. No known test offers complete assurance that they are free of micro-organism. Use only the appropriate rotors and adapters. Biosafe hoods should be used when Risk Group II materials as identified in the World Health Organization Laboratory Biosafety Manual are handled; materials of a higher group require more than one level of protection.
Ensure that the power cord is plugged into a wall receptacle that provides the correct voltage. Install the rotor or rotor yoke. Be sure the base is seated on the drive shaft, with the pin in the rotor groove. The shaft can be damaged if the rotor is dropped onto it or forced sideways. The rotor is ready to load. You can load fixed angle rotors either before or after installing the rotor in the centrifuge.
Load the rotor according to the rotor manual. If a tie-down nut comes off the shaft during centrifugation, the plastic nut furnished will break apart in the chamber, causing minimal damage to the centrifuge. A loose metal object could substantially damage the rotor, chamber, and lid, and could potentially escape the chamber into the laboratory, causing personal injury or property damage.
Firmly fasten the tie-down nut on the shaft using the torquing bar Do not overtighten the nut. If you are using this rotor, install the lid and turn the knob to the right clockwise until firmly fastened on the shaft. Close the door. A counter will record how many seconds the centrifuge has been active. To stop the centrifuge, simply let go of the button and the centrifuge will slow down and come to a halt.
The lid automatically unlocks once the motor has stopped and it is safe to open. Wait until you hear a click from the lid that indicates the lock being opened. To use the Time Spin option, select the clock symbol using the orange click-dial. The G force and the timer can be adjusted by pushing and rotating the orange click-dial while holding it down.
Clockwise rotations will increase the value, anti-clockwise rotations will decrease it. Click the confirm button to start the run. When the centrifuge is running with a timer, the remaining time is displayed on the top right position 1. The clock button 2 allows for the timer to be adjusted, and the cancel button 3 can be used to switch off the centrifuge manually. The centrifuge is intended for the separation of suspensions and solutions held in the appropriate 1. We use cookies to improve your browsing experience on our website.
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